De Vos was born in Hulst near Antwerp, now in the Dutch province of Zeeland. Like his older brother Cornelis and younger brother Jan, he studied under the little-known painter David Remeeus (1559–1626). He specialized in monumental animal scenes, especially hunts for aristocratic patrons, that are heavily influenced by Frans Snyders (to whom his sister Margaretha was married). De Vos became a master and joined the guild of St. Luke in 1620.
As was frequent amongst artists in Antwerp, De Vos frequently collaborated with other painters. He painted animals in hunting scenes and armor in mythologies by Peter Paul Rubens and his studio. He also worked with Thomas Willeboirts Bosschaert, Erasmus Quellinus II, Anthony van Dyck, and Jan Wildens